human intervertebral disc nucleus pulposus cells  (Procell Inc)

Journal: Journal of Inflammation Research

Article Title: Single-Cell Analysis Reveals Aspirin Restores Intervertebral Disc Integrity via Ferroptosis Regulation

doi: 10.2147/JIR.S519218

Figure Lengend Snippet: ( A ) Expression levels of ferroptosis inhibitory gene GPX4 and ferroptosis-activating genes CHAC1, TGFBR1, SMAD7, and CTSB at different stages. ( B ) Cell viability of human intervertebral disc nucleus pulposus (NPC) cells treated with varying concentrations (1, 5, and 10 mg/L) of LPS for 48 hours, measured by CCK-8 assay. ( C ) Representative PE fluorescence histograms of NPCs showing necrotic cells stained with PI, along with the quantitative results of necrotic percentages. ( D ) Representative fluorescent images depicting lipid peroxidation levels in NPCs, stained green with BODIPY, and nuclei stained blue with Hoechst 33342. ( E ) Representative FITC fluorescence histograms of NPCs showing cytosolic ROS production using DCFH-DA dye, with quantitative analysis of mean fluorescence intensity (MFI). *p < 0.05, ***p < 0.001 and ****p < 0.0001.

Article Snippet: Human intervertebral disc nucleus pulposus cells were purchased from Procell (#CP-H097, Wuhan, China) and passed to the third generation for experimental purposes.

Techniques: Expressing, CCK-8 Assay, Fluorescence, Staining

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: Bioinformatics analysis of degenerated human lumbar nucleus pulposus cells of intervertebral discs compared with normal nucleus pulposus cells. A: Heat map miRNA; B: Volcano map displays differentially expressed mRNA; C:Bubble map displays KEGG enrichment signal pathway analysis results; D:miR-503-gene-pathway network map construction; E: Venn map clarifies the common gene (TNIK) between TargetScan and Wnt signaling pathway and miR-503 target differential genes.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques:

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: Human nucleus pulposus cells miR-503 of degenerated intervertebral disc and targeted differentially expressed mRNA.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques:

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: Changes in the activity and function human degenerated intervertebral discs nucleus pulposus cells. A:CCK8 detects the changes of cell viability; B: ELISA detects the expression of apoptosis-related protein Bax; C, D, E: RT-PCR detects the expression changes of col-2, wnt, miR-503 in the cells.*P < 0.05.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: Changes in the activity and function human degenerated intervertebral discs nucleus pulposus cells. A: Western blot technology detects type II collagen expression as well as Wnt expression in the two groups of cells. B, C: The expression of col-2 and wnt in cells was analyzed by western blot; D, E: The proliferation ability and apoptosis of human degenerated intervertebral discs nucleus pulposus cells were detected by ELISA. * P<0.05.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques: Activity Assay, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: The effect of miR-503/TNIK on the function as well as activity of human nucleus pulposus cells of degenerated intervertebral discs. A: CCK8 detects cell activity; B: The expression of Bax was detected by ELISA; C, D: Dual luciferase gene reporter was used to detecte the targeted binding of miR-503 to TNIK; E, F, G: RT-PCR was used to detect the expression of miR-503, col-2 and wnt in cells. *P < 0.05.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques: Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Luciferase, Binding Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: miR-503 regulates the function and activity of human nucleus pulposus cells of degenerated intervertebral disc through Wnt. A: CCK8 was used to detect cell activity; B: ELISA was used to detect apoptosis-related protein Bax expression level; C, D, E: RT-PCR was used to detecte the expression of Col-2, Wnt and miR-503 in each group of cells.* P < 0.05.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: Western blot technology verifies that miR-503 regulates the function of human nucleus pulposus cells of degenerated intervertebral disc through Wnt. Western blot was used to detect the expression of col-2 and wnt in cells of each group. B, C. Western blot results showed the expression of col-2 and wnt after overexpression of miR-503, activation of Wnt, and overexpression of miR-503 combined with activation of wnt in degenerated human intervertebral disc nucleus pulposus cells. * P<0.05.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques: Western Blot, Expressing, Over Expression, Activation Assay

Journal: Journal of Musculoskeletal & Neuronal Interactions

Article Title: Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

doi:

Figure Lengend Snippet: Research hypothesis. miR-503 regulates the activity and function of degenerated human lumbar nucleus pulposus cells of intervertebral disc through targeted binding with TNIK to inhibit the Wnt signaling pathway.

Article Snippet: Human nucleus pulposus cells of the intervertebral disc were purchased from Procell, China.

Techniques: Activity Assay, Binding Assay